peripheral blood isolation kit Search Results


human peripheral blood neutrophil isolation kit  (Beijing Solarbio Science)
95
Beijing Solarbio Science human peripheral blood neutrophil isolation kit
The levels of NETs increase in HCC patients, especially in advanced and metastatic stages. A A heatmap illustrating the differential expression patterns of NETs-related genes in normal and HCC samples based on TCGA database analysis. B Box plots depicting the expression differences of NETs-related genes between normal and HCC samples from the TCGA database. C , D Representative images of HE (Hematoxylin and eosin) and IF staining for CD66b (C) and CitH3 (D) in tissue sections from HCs and HCC patients. The mean IOD of CD66b or CitH3 was quantified (Image Pro Plus) from three random regions per section (HC, n = 5; HCC, n = 5) and shown as fold change relative to HCs. E Representative images of IF staining for CitH3 in <t>peripheral</t> blood <t>neutrophils</t> from HC and HCC patient F Western blot analysis of CitH3 expression in peripheral blood neutrophils from HCs ( n = 8) and HCC ( n = 8) patients, with band intensities normalized to β-actin and shown as fold change relative to controls G ELISA analysis of serum MPO-DNA levels in HCs and HCC patients. H ELISA analysis of serum MPO-DNA levels in HCC patients at different TNM stages (I–IV). I ELISA analysis of serum MPO-DNA levels in HCC patients with and without metastasis. J ELISA analysis of serum MPO-DNA levels in HCC patients with intrahepatic metastasis and extrahepatic metastasis. K Western blot analysis of CitH3 expression in peripheral blood neutrophils from HCC patients with ( n = 6) and without ( n = 6) extrahepatic metastasis. Band intensities were normalized to β-actin and quantified relative to the non-metastasis group White and black scale bars: 50 μm. Data are presented as median (IQR) (G-J) and other data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001. Student’s t test (C, D, F, K), Mann–Whitney U test (B, G, I, J), Kruskal–Wallis test (H)
Human Peripheral Blood Neutrophil Isolation Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/peripheral+blood+isolation+kit/Human+Peripheral+Blood+Neutrophil+Isolation+Solution+Kit/pmc12853889-112-15-22
Average 95 stars, based on 1 article reviews
human peripheral blood neutrophil isolation kit - by Bioz Stars, 2026-09
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pig peripheral blood lymphocyte separation solution kit  (Beijing Solarbio Science)
93
Beijing Solarbio Science pig peripheral blood lymphocyte separation solution kit
The levels of NETs increase in HCC patients, especially in advanced and metastatic stages. A A heatmap illustrating the differential expression patterns of NETs-related genes in normal and HCC samples based on TCGA database analysis. B Box plots depicting the expression differences of NETs-related genes between normal and HCC samples from the TCGA database. C , D Representative images of HE (Hematoxylin and eosin) and IF staining for CD66b (C) and CitH3 (D) in tissue sections from HCs and HCC patients. The mean IOD of CD66b or CitH3 was quantified (Image Pro Plus) from three random regions per section (HC, n = 5; HCC, n = 5) and shown as fold change relative to HCs. E Representative images of IF staining for CitH3 in <t>peripheral</t> blood <t>neutrophils</t> from HC and HCC patient F Western blot analysis of CitH3 expression in peripheral blood neutrophils from HCs ( n = 8) and HCC ( n = 8) patients, with band intensities normalized to β-actin and shown as fold change relative to controls G ELISA analysis of serum MPO-DNA levels in HCs and HCC patients. H ELISA analysis of serum MPO-DNA levels in HCC patients at different TNM stages (I–IV). I ELISA analysis of serum MPO-DNA levels in HCC patients with and without metastasis. J ELISA analysis of serum MPO-DNA levels in HCC patients with intrahepatic metastasis and extrahepatic metastasis. K Western blot analysis of CitH3 expression in peripheral blood neutrophils from HCC patients with ( n = 6) and without ( n = 6) extrahepatic metastasis. Band intensities were normalized to β-actin and quantified relative to the non-metastasis group White and black scale bars: 50 μm. Data are presented as median (IQR) (G-J) and other data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001. Student’s t test (C, D, F, K), Mann–Whitney U test (B, G, I, J), Kruskal–Wallis test (H)
Pig Peripheral Blood Lymphocyte Separation Solution Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/peripheral+blood+isolation+kit/Pig+peripheral+blood+platelets+isolation+kit/pmc09781062-88-5-12
Average 93 stars, based on 1 article reviews
pig peripheral blood lymphocyte separation solution kit - by Bioz Stars, 2026-09
93/100 stars
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peripheral blood mononuclear cells pbmcs  (Beijing Solarbio Science)
95
Beijing Solarbio Science peripheral blood mononuclear cells pbmcs
Effect of PEG-Liker (PEG-IALLIPF), Trp, or MPP-Trp on the production of NO and Pro-inflammatory cytokines. ( A ) In <t>Control-PBMCs,</t> Asthma-PBMCs, Asthma-PBMCs + PEG-Liker, Asthma-PBMCs + Trp and Asthma-PBMCs + MPP-Trp group, NO production were examined by NO assay kit. Pro-inflammatory cytokines ( B ) TNF-α, ( C ) IL-1β, and ( D ) IL-6 contents in all groups were examined by ELISA assay. Data were presented as mean ± SD of three independent experiments. * P <0.05, ** P <0.01, *** P <0.001 vs Asthma-PBMCs group.
Peripheral Blood Mononuclear Cells Pbmcs, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/peripheral+blood+isolation+kit/Mouse+Peripheral+Blood+Mononuclear+Cell+Isolation+Solution+Kit/pmc11317058-70-2-29
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peripheral blood mononuclear cells pbmcs - by Bioz Stars, 2026-09
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peripheral blood monocyte isolation solution kit  (Beijing Solarbio Science)
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Beijing Solarbio Science peripheral blood monocyte isolation solution kit
Figure 7. DCD is beneficial to AIS recovery and DCD-containing BM-MSC-derived migrasome effectively promotes phagocytosis of macrophages. A– G) <t>Peripheral</t> blood of AIS patients (acute phase, 0–3d after disease onset, n = 16) and healthy controls (HC, n = 8) were collected. (A) Plasma DCD concentration was assessed with ELISA. *p < 0.05, compared with HC by Student’s t-test (mean ± standard deviation). (B) Correlation of clinic parameters and plasma DCD concentration was assessed with Spearman correlation analysis and Point-biserial correlations. *p < 0.05. DM, diabetes mellitus, CHD, coronary heart disease. (C) Representative images of the magnetic resonance diffusion weighted imaging (MR-DWI) of AIS patients with low plasma DCD concentration (DCD ≤3.33 ng ml−1) or high plasma DCD concentration (DCD > 3.33 ng ml−1). (D) Association between plasma DCD concentration with infarct scale was estimated with Spearman correlation analysis. (E) Association between plasma DCD concentration with delta NIHSS (NIHSS at 7d minus NIHSS at 1d) was estimated with Spearman correlation analysis. (F) Representative images of the chest Computed Tomography (CT) of AIS patients with low plasma DCD concentration (DCD ≤3.39 ng ml−1, median of the cohort) or high plasma DCD concentration (DCD > 3.39 ng ml−1, median of the cohort). (G) Pie charts showing the occurrence of post-stroke pneumonia in AIS patients with low and high plasma DCD concentrations. H) DCD (1 ng ml−1), PBS-migrasomes (PBS-M, 50 μg ml−1) or E. Coli-migrasomes (E. Coli-M, 50 μg ml−1) labeled with Dil (red) were treated to BMDM (15 min). Immunostaining of WGA (green) and DCD (withe) in migrasome-treated BMDM was performed. Experiments were repeated for three times. I,J) BMDM were first pre-stimulated with DCD (1 ng ml−1), PBS-M (50 μg ml−1), or E. Coli-M (50 μg ml−1) for overnight then treated with E. Coli (E. Coli : BMDM = 20:1, 1 h). Phagocytic efficiency of BMDM to GFP expressing E. Coli was assessed with flow cytometry (I) and immunostaining (J). Experiments were repeated three times. **p < 0.01, compared with PBS-treated group by one-way ANOVA (mean ± standard deviation).
Peripheral Blood Monocyte Isolation Solution Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/peripheral+blood+isolation+kit/Human+Peripheral+Blood+Monocyte+Isolation+Solution+Kit/pm37246283-348-21-27
Average 94 stars, based on 1 article reviews
peripheral blood monocyte isolation solution kit - by Bioz Stars, 2026-09
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bovine peripheral blood neutrophils isolation kit  (Beijing Solarbio Science)
93
Beijing Solarbio Science bovine peripheral blood neutrophils isolation kit
Figure 7. DCD is beneficial to AIS recovery and DCD-containing BM-MSC-derived migrasome effectively promotes phagocytosis of macrophages. A– G) <t>Peripheral</t> blood of AIS patients (acute phase, 0–3d after disease onset, n = 16) and healthy controls (HC, n = 8) were collected. (A) Plasma DCD concentration was assessed with ELISA. *p < 0.05, compared with HC by Student’s t-test (mean ± standard deviation). (B) Correlation of clinic parameters and plasma DCD concentration was assessed with Spearman correlation analysis and Point-biserial correlations. *p < 0.05. DM, diabetes mellitus, CHD, coronary heart disease. (C) Representative images of the magnetic resonance diffusion weighted imaging (MR-DWI) of AIS patients with low plasma DCD concentration (DCD ≤3.33 ng ml−1) or high plasma DCD concentration (DCD > 3.33 ng ml−1). (D) Association between plasma DCD concentration with infarct scale was estimated with Spearman correlation analysis. (E) Association between plasma DCD concentration with delta NIHSS (NIHSS at 7d minus NIHSS at 1d) was estimated with Spearman correlation analysis. (F) Representative images of the chest Computed Tomography (CT) of AIS patients with low plasma DCD concentration (DCD ≤3.39 ng ml−1, median of the cohort) or high plasma DCD concentration (DCD > 3.39 ng ml−1, median of the cohort). (G) Pie charts showing the occurrence of post-stroke pneumonia in AIS patients with low and high plasma DCD concentrations. H) DCD (1 ng ml−1), PBS-migrasomes (PBS-M, 50 μg ml−1) or E. Coli-migrasomes (E. Coli-M, 50 μg ml−1) labeled with Dil (red) were treated to BMDM (15 min). Immunostaining of WGA (green) and DCD (withe) in migrasome-treated BMDM was performed. Experiments were repeated for three times. I,J) BMDM were first pre-stimulated with DCD (1 ng ml−1), PBS-M (50 μg ml−1), or E. Coli-M (50 μg ml−1) for overnight then treated with E. Coli (E. Coli : BMDM = 20:1, 1 h). Phagocytic efficiency of BMDM to GFP expressing E. Coli was assessed with flow cytometry (I) and immunostaining (J). Experiments were repeated three times. **p < 0.01, compared with PBS-treated group by one-way ANOVA (mean ± standard deviation).
Bovine Peripheral Blood Neutrophils Isolation Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/peripheral+blood+isolation+kit/Bovine+Peripheral+Blood+Neutrophil+Isolation+Solution+Kit/pm35151471-54-10-23
Average 93 stars, based on 1 article reviews
bovine peripheral blood neutrophils isolation kit - by Bioz Stars, 2026-09
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canine peripheral blood mononuclear cell extraction kit  (Beijing Solarbio Science)
95
Beijing Solarbio Science canine peripheral blood mononuclear cell extraction kit
Figure 7. DCD is beneficial to AIS recovery and DCD-containing BM-MSC-derived migrasome effectively promotes phagocytosis of macrophages. A– G) <t>Peripheral</t> blood of AIS patients (acute phase, 0–3d after disease onset, n = 16) and healthy controls (HC, n = 8) were collected. (A) Plasma DCD concentration was assessed with ELISA. *p < 0.05, compared with HC by Student’s t-test (mean ± standard deviation). (B) Correlation of clinic parameters and plasma DCD concentration was assessed with Spearman correlation analysis and Point-biserial correlations. *p < 0.05. DM, diabetes mellitus, CHD, coronary heart disease. (C) Representative images of the magnetic resonance diffusion weighted imaging (MR-DWI) of AIS patients with low plasma DCD concentration (DCD ≤3.33 ng ml−1) or high plasma DCD concentration (DCD > 3.33 ng ml−1). (D) Association between plasma DCD concentration with infarct scale was estimated with Spearman correlation analysis. (E) Association between plasma DCD concentration with delta NIHSS (NIHSS at 7d minus NIHSS at 1d) was estimated with Spearman correlation analysis. (F) Representative images of the chest Computed Tomography (CT) of AIS patients with low plasma DCD concentration (DCD ≤3.39 ng ml−1, median of the cohort) or high plasma DCD concentration (DCD > 3.39 ng ml−1, median of the cohort). (G) Pie charts showing the occurrence of post-stroke pneumonia in AIS patients with low and high plasma DCD concentrations. H) DCD (1 ng ml−1), PBS-migrasomes (PBS-M, 50 μg ml−1) or E. Coli-migrasomes (E. Coli-M, 50 μg ml−1) labeled with Dil (red) were treated to BMDM (15 min). Immunostaining of WGA (green) and DCD (withe) in migrasome-treated BMDM was performed. Experiments were repeated for three times. I,J) BMDM were first pre-stimulated with DCD (1 ng ml−1), PBS-M (50 μg ml−1), or E. Coli-M (50 μg ml−1) for overnight then treated with E. Coli (E. Coli : BMDM = 20:1, 1 h). Phagocytic efficiency of BMDM to GFP expressing E. Coli was assessed with flow cytometry (I) and immunostaining (J). Experiments were repeated three times. **p < 0.01, compared with PBS-treated group by one-way ANOVA (mean ± standard deviation).
Canine Peripheral Blood Mononuclear Cell Extraction Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/peripheral+blood+isolation+kit/Camel+Peripheral+Blood+Mononuclear+Cell+Isolation+Solution+Kit/pmc09513111-68-7-14
Average 95 stars, based on 1 article reviews
canine peripheral blood mononuclear cell extraction kit - by Bioz Stars, 2026-09
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rat peripheral blood lymphocyte isolation kit p8630  (Beijing Solarbio Science)
93
Beijing Solarbio Science rat peripheral blood lymphocyte isolation kit p8630
Figure 7. DCD is beneficial to AIS recovery and DCD-containing BM-MSC-derived migrasome effectively promotes phagocytosis of macrophages. A– G) <t>Peripheral</t> blood of AIS patients (acute phase, 0–3d after disease onset, n = 16) and healthy controls (HC, n = 8) were collected. (A) Plasma DCD concentration was assessed with ELISA. *p < 0.05, compared with HC by Student’s t-test (mean ± standard deviation). (B) Correlation of clinic parameters and plasma DCD concentration was assessed with Spearman correlation analysis and Point-biserial correlations. *p < 0.05. DM, diabetes mellitus, CHD, coronary heart disease. (C) Representative images of the magnetic resonance diffusion weighted imaging (MR-DWI) of AIS patients with low plasma DCD concentration (DCD ≤3.33 ng ml−1) or high plasma DCD concentration (DCD > 3.33 ng ml−1). (D) Association between plasma DCD concentration with infarct scale was estimated with Spearman correlation analysis. (E) Association between plasma DCD concentration with delta NIHSS (NIHSS at 7d minus NIHSS at 1d) was estimated with Spearman correlation analysis. (F) Representative images of the chest Computed Tomography (CT) of AIS patients with low plasma DCD concentration (DCD ≤3.39 ng ml−1, median of the cohort) or high plasma DCD concentration (DCD > 3.39 ng ml−1, median of the cohort). (G) Pie charts showing the occurrence of post-stroke pneumonia in AIS patients with low and high plasma DCD concentrations. H) DCD (1 ng ml−1), PBS-migrasomes (PBS-M, 50 μg ml−1) or E. Coli-migrasomes (E. Coli-M, 50 μg ml−1) labeled with Dil (red) were treated to BMDM (15 min). Immunostaining of WGA (green) and DCD (withe) in migrasome-treated BMDM was performed. Experiments were repeated for three times. I,J) BMDM were first pre-stimulated with DCD (1 ng ml−1), PBS-M (50 μg ml−1), or E. Coli-M (50 μg ml−1) for overnight then treated with E. Coli (E. Coli : BMDM = 20:1, 1 h). Phagocytic efficiency of BMDM to GFP expressing E. Coli was assessed with flow cytometry (I) and immunostaining (J). Experiments were repeated three times. **p < 0.01, compared with PBS-treated group by one-way ANOVA (mean ± standard deviation).
Rat Peripheral Blood Lymphocyte Isolation Kit P8630, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/peripheral+blood+isolation+kit/Rat+Peripheral+Blood+Lymphocyte+Isolation+Solution+Kit/10__2147_slash_jir__s346365-53-0-18
Average 93 stars, based on 1 article reviews
rat peripheral blood lymphocyte isolation kit p8630 - by Bioz Stars, 2026-09
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mouse peripheral blood lymphocyte isolation kit  (Beijing Solarbio Science)
93
Beijing Solarbio Science mouse peripheral blood lymphocyte isolation kit
Figure 7. DCD is beneficial to AIS recovery and DCD-containing BM-MSC-derived migrasome effectively promotes phagocytosis of macrophages. A– G) <t>Peripheral</t> blood of AIS patients (acute phase, 0–3d after disease onset, n = 16) and healthy controls (HC, n = 8) were collected. (A) Plasma DCD concentration was assessed with ELISA. *p < 0.05, compared with HC by Student’s t-test (mean ± standard deviation). (B) Correlation of clinic parameters and plasma DCD concentration was assessed with Spearman correlation analysis and Point-biserial correlations. *p < 0.05. DM, diabetes mellitus, CHD, coronary heart disease. (C) Representative images of the magnetic resonance diffusion weighted imaging (MR-DWI) of AIS patients with low plasma DCD concentration (DCD ≤3.33 ng ml−1) or high plasma DCD concentration (DCD > 3.33 ng ml−1). (D) Association between plasma DCD concentration with infarct scale was estimated with Spearman correlation analysis. (E) Association between plasma DCD concentration with delta NIHSS (NIHSS at 7d minus NIHSS at 1d) was estimated with Spearman correlation analysis. (F) Representative images of the chest Computed Tomography (CT) of AIS patients with low plasma DCD concentration (DCD ≤3.39 ng ml−1, median of the cohort) or high plasma DCD concentration (DCD > 3.39 ng ml−1, median of the cohort). (G) Pie charts showing the occurrence of post-stroke pneumonia in AIS patients with low and high plasma DCD concentrations. H) DCD (1 ng ml−1), PBS-migrasomes (PBS-M, 50 μg ml−1) or E. Coli-migrasomes (E. Coli-M, 50 μg ml−1) labeled with Dil (red) were treated to BMDM (15 min). Immunostaining of WGA (green) and DCD (withe) in migrasome-treated BMDM was performed. Experiments were repeated for three times. I,J) BMDM were first pre-stimulated with DCD (1 ng ml−1), PBS-M (50 μg ml−1), or E. Coli-M (50 μg ml−1) for overnight then treated with E. Coli (E. Coli : BMDM = 20:1, 1 h). Phagocytic efficiency of BMDM to GFP expressing E. Coli was assessed with flow cytometry (I) and immunostaining (J). Experiments were repeated three times. **p < 0.01, compared with PBS-treated group by one-way ANOVA (mean ± standard deviation).
Mouse Peripheral Blood Lymphocyte Isolation Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/peripheral+blood+isolation+kit/Mouse+Peripheral+Blood+Lymphocyte+Isolation+Solution+Kit/10__1038_slash_s41522___025___00809___4-344-23-29
Average 93 stars, based on 1 article reviews
mouse peripheral blood lymphocyte isolation kit - by Bioz Stars, 2026-09
93/100 stars
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peripheral blood lymphocyte isolation solution kit  (Beijing Solarbio Science)
94
Beijing Solarbio Science peripheral blood lymphocyte isolation solution kit
Figure 7. DCD is beneficial to AIS recovery and DCD-containing BM-MSC-derived migrasome effectively promotes phagocytosis of macrophages. A– G) <t>Peripheral</t> blood of AIS patients (acute phase, 0–3d after disease onset, n = 16) and healthy controls (HC, n = 8) were collected. (A) Plasma DCD concentration was assessed with ELISA. *p < 0.05, compared with HC by Student’s t-test (mean ± standard deviation). (B) Correlation of clinic parameters and plasma DCD concentration was assessed with Spearman correlation analysis and Point-biserial correlations. *p < 0.05. DM, diabetes mellitus, CHD, coronary heart disease. (C) Representative images of the magnetic resonance diffusion weighted imaging (MR-DWI) of AIS patients with low plasma DCD concentration (DCD ≤3.33 ng ml−1) or high plasma DCD concentration (DCD > 3.33 ng ml−1). (D) Association between plasma DCD concentration with infarct scale was estimated with Spearman correlation analysis. (E) Association between plasma DCD concentration with delta NIHSS (NIHSS at 7d minus NIHSS at 1d) was estimated with Spearman correlation analysis. (F) Representative images of the chest Computed Tomography (CT) of AIS patients with low plasma DCD concentration (DCD ≤3.39 ng ml−1, median of the cohort) or high plasma DCD concentration (DCD > 3.39 ng ml−1, median of the cohort). (G) Pie charts showing the occurrence of post-stroke pneumonia in AIS patients with low and high plasma DCD concentrations. H) DCD (1 ng ml−1), PBS-migrasomes (PBS-M, 50 μg ml−1) or E. Coli-migrasomes (E. Coli-M, 50 μg ml−1) labeled with Dil (red) were treated to BMDM (15 min). Immunostaining of WGA (green) and DCD (withe) in migrasome-treated BMDM was performed. Experiments were repeated for three times. I,J) BMDM were first pre-stimulated with DCD (1 ng ml−1), PBS-M (50 μg ml−1), or E. Coli-M (50 μg ml−1) for overnight then treated with E. Coli (E. Coli : BMDM = 20:1, 1 h). Phagocytic efficiency of BMDM to GFP expressing E. Coli was assessed with flow cytometry (I) and immunostaining (J). Experiments were repeated three times. **p < 0.01, compared with PBS-treated group by one-way ANOVA (mean ± standard deviation).
Peripheral Blood Lymphocyte Isolation Solution Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/peripheral+blood+isolation+kit/Camel+Peripheral+Blood+Lymphocyte+Isolation+Solution+Kit/pm41461131-138-8-14
Average 94 stars, based on 1 article reviews
peripheral blood lymphocyte isolation solution kit - by Bioz Stars, 2026-09
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mouse peripheral blood monocytes pbmcs  (Beijing Solarbio Science)
94
Beijing Solarbio Science mouse peripheral blood monocytes pbmcs
( A ) UMAP plot of monocyte clusters in WT lungs ( n = 3) and CLEC5A −/− lungs ( n = 3) and percentage in immune cells. ( B ) Dot plot showing the expression of adhesion molecules in each subtype of endothelial cells. ( C ) GSEA of the DEGs in the endothelial cells between CLEC5A −/− and WT lungs showing significant enrichment of cell-adhesion related-GO pathways, in which the up-regulated DEGs were mainly enriched. The significance was determined by P value with false discovery rate (FDR) < 0.25. ( D ) Mouse PMVECs were isolated and challenged by LPS (10 μg/ml) for 24 hours. ( E ) Levels of MCP-1 and VCAM-1 in the supernatant by ELISA. ( F ) Relative mRNA expression of MCP-1 and VCAM-1 in PMVECs. ( G ) Overview of in vitro adhesion and trans-endothelial migration assays in mouse PMVECs and HUVECs. ( H ) Mouse <t>PBMCs</t> were labeled by BCECF-AM, and the adherent monocytes to PMVECs were observed under a fluorescence microscopy. Scale bar, 50 μm. Monocyte-endothelial adhesion was quantified as the percentage of fluorescence intensity relative to the WT group. ( I ) Transmigration of mouse PBMCs across the PMVEC monolayer was shown as the percentage of transmigrated cells relative to the WT group by MTT assay. Lentivirus-mediated gene delivery for CLEC5A (CLEC5A oe ) or shRNA targeting CLEC5A (CLEC5A sh ) were carried out in HUVECs. ( J ) Adhesion of THP-1 to HUVECs was shown as fluorescence and quantified as the percentage of fluorescence intensity relative to the control group. Scale bar, 50 μm. ( K ) Migration of THP-1 through the HUVEC monolayer shown as the percentage of transmigrated cells relative to the control group. The statistical significance was determined by an unpaired two-tailed t test (or with Welch’s correction) for comparison between two groups and by one-way ANOVA test for comparisons among four groups (biological replicates, n = 4 per group for each experiment).
Mouse Peripheral Blood Monocytes Pbmcs, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/peripheral+blood+isolation+kit/Mouse+Peripheral+Blood+Monocyte+Isolation+Solution+Kit/pmc12154197-407-0-14
Average 94 stars, based on 1 article reviews
mouse peripheral blood monocytes pbmcs - by Bioz Stars, 2026-09
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mouse peripheral blood neutrophil isolation kit  (Beijing Solarbio Science)
95
Beijing Solarbio Science mouse peripheral blood neutrophil isolation kit
( A ) UMAP plot of monocyte clusters in WT lungs ( n = 3) and CLEC5A −/− lungs ( n = 3) and percentage in immune cells. ( B ) Dot plot showing the expression of adhesion molecules in each subtype of endothelial cells. ( C ) GSEA of the DEGs in the endothelial cells between CLEC5A −/− and WT lungs showing significant enrichment of cell-adhesion related-GO pathways, in which the up-regulated DEGs were mainly enriched. The significance was determined by P value with false discovery rate (FDR) < 0.25. ( D ) Mouse PMVECs were isolated and challenged by LPS (10 μg/ml) for 24 hours. ( E ) Levels of MCP-1 and VCAM-1 in the supernatant by ELISA. ( F ) Relative mRNA expression of MCP-1 and VCAM-1 in PMVECs. ( G ) Overview of in vitro adhesion and trans-endothelial migration assays in mouse PMVECs and HUVECs. ( H ) Mouse <t>PBMCs</t> were labeled by BCECF-AM, and the adherent monocytes to PMVECs were observed under a fluorescence microscopy. Scale bar, 50 μm. Monocyte-endothelial adhesion was quantified as the percentage of fluorescence intensity relative to the WT group. ( I ) Transmigration of mouse PBMCs across the PMVEC monolayer was shown as the percentage of transmigrated cells relative to the WT group by MTT assay. Lentivirus-mediated gene delivery for CLEC5A (CLEC5A oe ) or shRNA targeting CLEC5A (CLEC5A sh ) were carried out in HUVECs. ( J ) Adhesion of THP-1 to HUVECs was shown as fluorescence and quantified as the percentage of fluorescence intensity relative to the control group. Scale bar, 50 μm. ( K ) Migration of THP-1 through the HUVEC monolayer shown as the percentage of transmigrated cells relative to the control group. The statistical significance was determined by an unpaired two-tailed t test (or with Welch’s correction) for comparison between two groups and by one-way ANOVA test for comparisons among four groups (biological replicates, n = 4 per group for each experiment).
Mouse Peripheral Blood Neutrophil Isolation Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/peripheral+blood+isolation+kit/Mouse+Peripheral+Blood+Neutrophil+Isolation+Solution+Kit/10__1016_slash_j__apsb__2025__09__011-164-17-23
Average 95 stars, based on 1 article reviews
mouse peripheral blood neutrophil isolation kit - by Bioz Stars, 2026-09
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peripheral blood lymphocyte separation kit  (Beijing Solarbio Science)
93
Beijing Solarbio Science peripheral blood lymphocyte separation kit
CD3 + T cells acquire PEDV from DCs and enter the <t>peripheral</t> blood. a After PEDV intranasal inoculation, blood samples were collected at the indicated time during which PBMCs were isolated and analyzed by FACS. The cells were gated based on CD3 + T cells (Q1), and gated cells were further selected based on the uptake of PEDV. b Quantification of the FACS results is shown in panel ( a ), n = 6 from 3 piglets per group. c , d PEDV-pulsed DCs were cocultured with CD3 + T cells to detect the transmission of DC-associated PEDV to CD3 + T cells. Then, PEDV transmission was quantified by analyzing the CD3 + PEDV + population, n = 3 wells per group. e Furthermore, after coculturing with CD3 + T cells for 2 h, DCs were separated by magnetic beads <t>separation</t> (MACS). The remaining CD3 + T cells remained in culture, and PEDV RNA expression levels were determined at the indicated time; the results of a representative experiment are shown, n = 3 per group. f FACS profiles of conjugate formation between CFSE-labeled T cells ( x -axis) and PKH26-labeled PEDV-pulsed DCs ( y -axis). Conjugates are apparent in the upper right quadrant, and the percentages of cells are shown. g Conjugation between CFSE-labeled T cells (green) and PKH26-labeled DCs (red) is indicated by CLSM, and the typical conjugation structure between the two cells is enlarged in panel ( a ). PEDV (blue) was detected in the conjugate structure using an antibody against N protein. Bars, 20 μm. h Electron micrographs of DC-T cell conjugates. PEDV-carrying DCs were cocultured with CD3 + T cells for 1 h and collected for TEM analysis. The sites of interaction between DCs and T cells showed firm interactions (black frame). DCs and T cell membranes were closely apposed at the tips of the protrusions (black asterisk). The fine ultrastructure of the virus particles (black arrowheads) was observed in the T cells adjacent to the conjugate structure. Bars, 2 μm. All data are the mean ± SD, comparisons performed with one-way ANOVA. * P < 0.05. The results are representative of three independent experiments
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The levels of NETs increase in HCC patients, especially in advanced and metastatic stages. A A heatmap illustrating the differential expression patterns of NETs-related genes in normal and HCC samples based on TCGA database analysis. B Box plots depicting the expression differences of NETs-related genes between normal and HCC samples from the TCGA database. C , D Representative images of HE (Hematoxylin and eosin) and IF staining for CD66b (C) and CitH3 (D) in tissue sections from HCs and HCC patients. The mean IOD of CD66b or CitH3 was quantified (Image Pro Plus) from three random regions per section (HC, n = 5; HCC, n = 5) and shown as fold change relative to HCs. E Representative images of IF staining for CitH3 in peripheral blood neutrophils from HC and HCC patient F Western blot analysis of CitH3 expression in peripheral blood neutrophils from HCs ( n = 8) and HCC ( n = 8) patients, with band intensities normalized to β-actin and shown as fold change relative to controls G ELISA analysis of serum MPO-DNA levels in HCs and HCC patients. H ELISA analysis of serum MPO-DNA levels in HCC patients at different TNM stages (I–IV). I ELISA analysis of serum MPO-DNA levels in HCC patients with and without metastasis. J ELISA analysis of serum MPO-DNA levels in HCC patients with intrahepatic metastasis and extrahepatic metastasis. K Western blot analysis of CitH3 expression in peripheral blood neutrophils from HCC patients with ( n = 6) and without ( n = 6) extrahepatic metastasis. Band intensities were normalized to β-actin and quantified relative to the non-metastasis group White and black scale bars: 50 μm. Data are presented as median (IQR) (G-J) and other data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001. Student’s t test (C, D, F, K), Mann–Whitney U test (B, G, I, J), Kruskal–Wallis test (H)

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Neutrophil-macrophage crosstalk via NETs–IL-17/VEGF/S100A9 axis promotes hepatocellular carcinoma progression

doi: 10.1186/s13046-025-03618-x

Figure Lengend Snippet: The levels of NETs increase in HCC patients, especially in advanced and metastatic stages. A A heatmap illustrating the differential expression patterns of NETs-related genes in normal and HCC samples based on TCGA database analysis. B Box plots depicting the expression differences of NETs-related genes between normal and HCC samples from the TCGA database. C , D Representative images of HE (Hematoxylin and eosin) and IF staining for CD66b (C) and CitH3 (D) in tissue sections from HCs and HCC patients. The mean IOD of CD66b or CitH3 was quantified (Image Pro Plus) from three random regions per section (HC, n = 5; HCC, n = 5) and shown as fold change relative to HCs. E Representative images of IF staining for CitH3 in peripheral blood neutrophils from HC and HCC patient F Western blot analysis of CitH3 expression in peripheral blood neutrophils from HCs ( n = 8) and HCC ( n = 8) patients, with band intensities normalized to β-actin and shown as fold change relative to controls G ELISA analysis of serum MPO-DNA levels in HCs and HCC patients. H ELISA analysis of serum MPO-DNA levels in HCC patients at different TNM stages (I–IV). I ELISA analysis of serum MPO-DNA levels in HCC patients with and without metastasis. J ELISA analysis of serum MPO-DNA levels in HCC patients with intrahepatic metastasis and extrahepatic metastasis. K Western blot analysis of CitH3 expression in peripheral blood neutrophils from HCC patients with ( n = 6) and without ( n = 6) extrahepatic metastasis. Band intensities were normalized to β-actin and quantified relative to the non-metastasis group White and black scale bars: 50 μm. Data are presented as median (IQR) (G-J) and other data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001. Student’s t test (C, D, F, K), Mann–Whitney U test (B, G, I, J), Kruskal–Wallis test (H)

Article Snippet: Neutrophils were isolated from the peripheral blood of healthy volunteers and HCC patients using the Human Peripheral Blood Neutrophil Isolation Kit (P9040, Solarbio, China), according to the manufacturer’s instructions.

Techniques: Quantitative Proteomics, Expressing, Staining, Western Blot, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

Activation of IL-17R/NF-κB signaling by NETs-attached IL-17 is responsible for NETs-induced M2 macrophage polarization. A Schematic illustration of the transcriptome sequencing protocol for THP1-M0 under three treatment conditions. The volcano plot shows significantly DEGs for Group B vs. A, Group C vs. A, and Group C vs. B. B KEGG pathway enrichment analysis of DEGs was conducted using the DAVID tool. C FCM analysis of the proportion of CD68 + CD206 + M2 derived from THP-1-M0 following 48-hour co-culture of HuH-7 cells with NETs and (or) AMG827. Quantification of the fold change in the proportion from three independent experiments is shown in the right panel. D Western blot analysis of IL-10 and VEGF expression in cell lysates following the same treatments as in C. Densitometric values were normalized to β-actin and compared to the control group, with quantification shown in the right panel E Western blot analysis of IL-17 expression in extracted and purified NETs from peripheral blood neutrophils of HCs ( n = 8) and HCC patients ( n = 8). F ELISA analysis of IL-17 levels carried by NETs standardized to 50 ng/µL in HCs ( n = 8) and patients with HCC ( n = 8). G Western blot analysis of IL-17 expression in peripheral blood neutrophils from HCs ( n = 8) and HCC ( n = 8) patients. Densitometric values were normalized to β-actin and shown as a fold change relative to HCs. H Representative IF images of IL-17 and NE staining in peripheral blood neutrophils with (lower panel) or without PMA stimulation (upper panel) for 4 h from HCs and HCC patients. I Western blot analysis was conducted to assess the expression levels of p-65 and p-p65(S536) in THP-1-M0 following 48 h co-culture of HuH-7 cells with NETs and (or) AMG827. Densitometric values were normalized to β-actin and shown as fold changes relative to control in the right panel. J FCM was used to analyze the proportion of CD68 + CD206 + M2 derived from THP-1-M0 following 48-hour co-culture of HuH-7 cells with NETs and (or) BAY11-7082. Quantification of the fold change in the proportion from three independent experiments is shown in the right panel. K IF analysis of CD206 + M2 macrophage from the above co-culture systems of J treatment for 48 h. L ELISA analysis of secretion levels of M2d-related cytokines VEGF, IL-10 and TGF-β, in the culture supernatant from the above co-culture systems of J treatment for 48 h White scale bars: 20 μm. Data are presented as mean ± SD. Ns, not significant. * p < 0.05, ** p < 0.01, *** p < 0.001. One-way ANOVA followed by the Newman-Keuls multiple comparison test (C, D, I, J, L); Student’s t test (F, G)

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Neutrophil-macrophage crosstalk via NETs–IL-17/VEGF/S100A9 axis promotes hepatocellular carcinoma progression

doi: 10.1186/s13046-025-03618-x

Figure Lengend Snippet: Activation of IL-17R/NF-κB signaling by NETs-attached IL-17 is responsible for NETs-induced M2 macrophage polarization. A Schematic illustration of the transcriptome sequencing protocol for THP1-M0 under three treatment conditions. The volcano plot shows significantly DEGs for Group B vs. A, Group C vs. A, and Group C vs. B. B KEGG pathway enrichment analysis of DEGs was conducted using the DAVID tool. C FCM analysis of the proportion of CD68 + CD206 + M2 derived from THP-1-M0 following 48-hour co-culture of HuH-7 cells with NETs and (or) AMG827. Quantification of the fold change in the proportion from three independent experiments is shown in the right panel. D Western blot analysis of IL-10 and VEGF expression in cell lysates following the same treatments as in C. Densitometric values were normalized to β-actin and compared to the control group, with quantification shown in the right panel E Western blot analysis of IL-17 expression in extracted and purified NETs from peripheral blood neutrophils of HCs ( n = 8) and HCC patients ( n = 8). F ELISA analysis of IL-17 levels carried by NETs standardized to 50 ng/µL in HCs ( n = 8) and patients with HCC ( n = 8). G Western blot analysis of IL-17 expression in peripheral blood neutrophils from HCs ( n = 8) and HCC ( n = 8) patients. Densitometric values were normalized to β-actin and shown as a fold change relative to HCs. H Representative IF images of IL-17 and NE staining in peripheral blood neutrophils with (lower panel) or without PMA stimulation (upper panel) for 4 h from HCs and HCC patients. I Western blot analysis was conducted to assess the expression levels of p-65 and p-p65(S536) in THP-1-M0 following 48 h co-culture of HuH-7 cells with NETs and (or) AMG827. Densitometric values were normalized to β-actin and shown as fold changes relative to control in the right panel. J FCM was used to analyze the proportion of CD68 + CD206 + M2 derived from THP-1-M0 following 48-hour co-culture of HuH-7 cells with NETs and (or) BAY11-7082. Quantification of the fold change in the proportion from three independent experiments is shown in the right panel. K IF analysis of CD206 + M2 macrophage from the above co-culture systems of J treatment for 48 h. L ELISA analysis of secretion levels of M2d-related cytokines VEGF, IL-10 and TGF-β, in the culture supernatant from the above co-culture systems of J treatment for 48 h White scale bars: 20 μm. Data are presented as mean ± SD. Ns, not significant. * p < 0.05, ** p < 0.01, *** p < 0.001. One-way ANOVA followed by the Newman-Keuls multiple comparison test (C, D, I, J, L); Student’s t test (F, G)

Article Snippet: Neutrophils were isolated from the peripheral blood of healthy volunteers and HCC patients using the Human Peripheral Blood Neutrophil Isolation Kit (P9040, Solarbio, China), according to the manufacturer’s instructions.

Techniques: Activation Assay, Sequencing, Derivative Assay, Co-Culture Assay, Western Blot, Expressing, Control, Purification, Enzyme-linked Immunosorbent Assay, Staining, Comparison

Effect of PEG-Liker (PEG-IALLIPF), Trp, or MPP-Trp on the production of NO and Pro-inflammatory cytokines. ( A ) In Control-PBMCs, Asthma-PBMCs, Asthma-PBMCs + PEG-Liker, Asthma-PBMCs + Trp and Asthma-PBMCs + MPP-Trp group, NO production were examined by NO assay kit. Pro-inflammatory cytokines ( B ) TNF-α, ( C ) IL-1β, and ( D ) IL-6 contents in all groups were examined by ELISA assay. Data were presented as mean ± SD of three independent experiments. * P <0.05, ** P <0.01, *** P <0.001 vs Asthma-PBMCs group.

Journal: International Journal of Nanomedicine

Article Title: Cathepsin B-Activatable Bioactive Peptide Nanocarrier for High-Efficiency Immunotherapy of Asthma

doi: 10.2147/IJN.S455633

Figure Lengend Snippet: Effect of PEG-Liker (PEG-IALLIPF), Trp, or MPP-Trp on the production of NO and Pro-inflammatory cytokines. ( A ) In Control-PBMCs, Asthma-PBMCs, Asthma-PBMCs + PEG-Liker, Asthma-PBMCs + Trp and Asthma-PBMCs + MPP-Trp group, NO production were examined by NO assay kit. Pro-inflammatory cytokines ( B ) TNF-α, ( C ) IL-1β, and ( D ) IL-6 contents in all groups were examined by ELISA assay. Data were presented as mean ± SD of three independent experiments. * P <0.05, ** P <0.01, *** P <0.001 vs Asthma-PBMCs group.

Article Snippet: The total peripheral blood mononuclear cells (PBMCs) were isolated from the whole blood samples of control group and asthma group by mouse peripheral blood mononuclear cell isolation kit (P6340; Solarbio, Beijing, China) according to the manufacturer's protocol.

Techniques: Control, Enzyme-linked Immunosorbent Assay

MPP-Trp was closely correlated with the balanced Th1/Th2 level and Th1/Th2-type cytokine production. ( A ) In Control-PBMCs, Asthma-PBMCs, Asthma-PBMCs + PEG-Liker, Asthma-PBMCs + Trp and Asthma-PBMCs + MPP-Trp group, Th1 population (CD4+IFN-γ+) and Th2 population (CD4+IL-4+) were selected by flow cytometry assay. ( B ) Relative mRNA expressions of IFN-γ, IL-4, IL-13, and IL-5 in all groups were assessed by qRT-PCR. ( C ) The IFN-γ, IL-4, IL-13, and IL-5 contents in all groups were examined by ELISA assay. Data were presented as mean ± SD of three independent experiments. * P <0.05, ** P <0.01, *** P <0.001 vs Asthma-PBMCs group.

Journal: International Journal of Nanomedicine

Article Title: Cathepsin B-Activatable Bioactive Peptide Nanocarrier for High-Efficiency Immunotherapy of Asthma

doi: 10.2147/IJN.S455633

Figure Lengend Snippet: MPP-Trp was closely correlated with the balanced Th1/Th2 level and Th1/Th2-type cytokine production. ( A ) In Control-PBMCs, Asthma-PBMCs, Asthma-PBMCs + PEG-Liker, Asthma-PBMCs + Trp and Asthma-PBMCs + MPP-Trp group, Th1 population (CD4+IFN-γ+) and Th2 population (CD4+IL-4+) were selected by flow cytometry assay. ( B ) Relative mRNA expressions of IFN-γ, IL-4, IL-13, and IL-5 in all groups were assessed by qRT-PCR. ( C ) The IFN-γ, IL-4, IL-13, and IL-5 contents in all groups were examined by ELISA assay. Data were presented as mean ± SD of three independent experiments. * P <0.05, ** P <0.01, *** P <0.001 vs Asthma-PBMCs group.

Article Snippet: The total peripheral blood mononuclear cells (PBMCs) were isolated from the whole blood samples of control group and asthma group by mouse peripheral blood mononuclear cell isolation kit (P6340; Solarbio, Beijing, China) according to the manufacturer's protocol.

Techniques: Control, Flow Cytometry, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay

MPP-Trp altered cytokine gene expression and production in a concentration-dependent way. In Control-PBMCs, Asthma-PBMCs, and Asthma-PBMCs + MPP-Trp (10, 50, 100 and 200 μg/mL) group, ( A ) Th1/Th2 cytokine gene expressions (IFN-γ, IL-4, IL-13, and IL-5) were determined by RT-qPCR. ( B ) The cytokines (IFN-γ, IL-4, IL-13, and IL-5) levels in all groups were detected by ELISA. Data were presented as mean ± SD of three independent experiments. ** P <0.01, *** P <0.001 vs Control-PBMCs group; # P <0.05, ## P <0.01, ### P <0.001 vs Asthma-PBMCs group.

Journal: International Journal of Nanomedicine

Article Title: Cathepsin B-Activatable Bioactive Peptide Nanocarrier for High-Efficiency Immunotherapy of Asthma

doi: 10.2147/IJN.S455633

Figure Lengend Snippet: MPP-Trp altered cytokine gene expression and production in a concentration-dependent way. In Control-PBMCs, Asthma-PBMCs, and Asthma-PBMCs + MPP-Trp (10, 50, 100 and 200 μg/mL) group, ( A ) Th1/Th2 cytokine gene expressions (IFN-γ, IL-4, IL-13, and IL-5) were determined by RT-qPCR. ( B ) The cytokines (IFN-γ, IL-4, IL-13, and IL-5) levels in all groups were detected by ELISA. Data were presented as mean ± SD of three independent experiments. ** P <0.01, *** P <0.001 vs Control-PBMCs group; # P <0.05, ## P <0.01, ### P <0.001 vs Asthma-PBMCs group.

Article Snippet: The total peripheral blood mononuclear cells (PBMCs) were isolated from the whole blood samples of control group and asthma group by mouse peripheral blood mononuclear cell isolation kit (P6340; Solarbio, Beijing, China) according to the manufacturer's protocol.

Techniques: Gene Expression, Concentration Assay, Control, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay

MPP-Trp altered cytokine gene expression and production in a time-dependent way. ( A ) In Control-PBMCs, Asthma-PBMCs, and 100 μg/mL Asthma-PBMCs + MPP-Trp (6, 12, 24, and 48 h) group, IFN-γ, IL-4, IL-13, and IL-5 mRNA levels were determined by RT-qPCR. ( B ) The Th1/Th2-type cytokines (IFN-γ, IL-4, IL-13, and IL-5) productions in all group were examined by ELISA. Data were presented as mean ± SD of three independent experiments. *** P <0.001 vs Control-PBMCs group; # P <0.05; ## P <0.01; ### P <0.001 vs Asthma-PBMCs group.

Journal: International Journal of Nanomedicine

Article Title: Cathepsin B-Activatable Bioactive Peptide Nanocarrier for High-Efficiency Immunotherapy of Asthma

doi: 10.2147/IJN.S455633

Figure Lengend Snippet: MPP-Trp altered cytokine gene expression and production in a time-dependent way. ( A ) In Control-PBMCs, Asthma-PBMCs, and 100 μg/mL Asthma-PBMCs + MPP-Trp (6, 12, 24, and 48 h) group, IFN-γ, IL-4, IL-13, and IL-5 mRNA levels were determined by RT-qPCR. ( B ) The Th1/Th2-type cytokines (IFN-γ, IL-4, IL-13, and IL-5) productions in all group were examined by ELISA. Data were presented as mean ± SD of three independent experiments. *** P <0.001 vs Control-PBMCs group; # P <0.05; ## P <0.01; ### P <0.001 vs Asthma-PBMCs group.

Article Snippet: The total peripheral blood mononuclear cells (PBMCs) were isolated from the whole blood samples of control group and asthma group by mouse peripheral blood mononuclear cell isolation kit (P6340; Solarbio, Beijing, China) according to the manufacturer's protocol.

Techniques: Gene Expression, Control, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay

Figure 7. DCD is beneficial to AIS recovery and DCD-containing BM-MSC-derived migrasome effectively promotes phagocytosis of macrophages. A– G) Peripheral blood of AIS patients (acute phase, 0–3d after disease onset, n = 16) and healthy controls (HC, n = 8) were collected. (A) Plasma DCD concentration was assessed with ELISA. *p < 0.05, compared with HC by Student’s t-test (mean ± standard deviation). (B) Correlation of clinic parameters and plasma DCD concentration was assessed with Spearman correlation analysis and Point-biserial correlations. *p < 0.05. DM, diabetes mellitus, CHD, coronary heart disease. (C) Representative images of the magnetic resonance diffusion weighted imaging (MR-DWI) of AIS patients with low plasma DCD concentration (DCD ≤3.33 ng ml−1) or high plasma DCD concentration (DCD > 3.33 ng ml−1). (D) Association between plasma DCD concentration with infarct scale was estimated with Spearman correlation analysis. (E) Association between plasma DCD concentration with delta NIHSS (NIHSS at 7d minus NIHSS at 1d) was estimated with Spearman correlation analysis. (F) Representative images of the chest Computed Tomography (CT) of AIS patients with low plasma DCD concentration (DCD ≤3.39 ng ml−1, median of the cohort) or high plasma DCD concentration (DCD > 3.39 ng ml−1, median of the cohort). (G) Pie charts showing the occurrence of post-stroke pneumonia in AIS patients with low and high plasma DCD concentrations. H) DCD (1 ng ml−1), PBS-migrasomes (PBS-M, 50 μg ml−1) or E. Coli-migrasomes (E. Coli-M, 50 μg ml−1) labeled with Dil (red) were treated to BMDM (15 min). Immunostaining of WGA (green) and DCD (withe) in migrasome-treated BMDM was performed. Experiments were repeated for three times. I,J) BMDM were first pre-stimulated with DCD (1 ng ml−1), PBS-M (50 μg ml−1), or E. Coli-M (50 μg ml−1) for overnight then treated with E. Coli (E. Coli : BMDM = 20:1, 1 h). Phagocytic efficiency of BMDM to GFP expressing E. Coli was assessed with flow cytometry (I) and immunostaining (J). Experiments were repeated three times. **p < 0.01, compared with PBS-treated group by one-way ANOVA (mean ± standard deviation).

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia.

doi: 10.1002/advs.202206432

Figure Lengend Snippet: Figure 7. DCD is beneficial to AIS recovery and DCD-containing BM-MSC-derived migrasome effectively promotes phagocytosis of macrophages. A– G) Peripheral blood of AIS patients (acute phase, 0–3d after disease onset, n = 16) and healthy controls (HC, n = 8) were collected. (A) Plasma DCD concentration was assessed with ELISA. *p < 0.05, compared with HC by Student’s t-test (mean ± standard deviation). (B) Correlation of clinic parameters and plasma DCD concentration was assessed with Spearman correlation analysis and Point-biserial correlations. *p < 0.05. DM, diabetes mellitus, CHD, coronary heart disease. (C) Representative images of the magnetic resonance diffusion weighted imaging (MR-DWI) of AIS patients with low plasma DCD concentration (DCD ≤3.33 ng ml−1) or high plasma DCD concentration (DCD > 3.33 ng ml−1). (D) Association between plasma DCD concentration with infarct scale was estimated with Spearman correlation analysis. (E) Association between plasma DCD concentration with delta NIHSS (NIHSS at 7d minus NIHSS at 1d) was estimated with Spearman correlation analysis. (F) Representative images of the chest Computed Tomography (CT) of AIS patients with low plasma DCD concentration (DCD ≤3.39 ng ml−1, median of the cohort) or high plasma DCD concentration (DCD > 3.39 ng ml−1, median of the cohort). (G) Pie charts showing the occurrence of post-stroke pneumonia in AIS patients with low and high plasma DCD concentrations. H) DCD (1 ng ml−1), PBS-migrasomes (PBS-M, 50 μg ml−1) or E. Coli-migrasomes (E. Coli-M, 50 μg ml−1) labeled with Dil (red) were treated to BMDM (15 min). Immunostaining of WGA (green) and DCD (withe) in migrasome-treated BMDM was performed. Experiments were repeated for three times. I,J) BMDM were first pre-stimulated with DCD (1 ng ml−1), PBS-M (50 μg ml−1), or E. Coli-M (50 μg ml−1) for overnight then treated with E. Coli (E. Coli : BMDM = 20:1, 1 h). Phagocytic efficiency of BMDM to GFP expressing E. Coli was assessed with flow cytometry (I) and immunostaining (J). Experiments were repeated three times. **p < 0.01, compared with PBS-treated group by one-way ANOVA (mean ± standard deviation).

Article Snippet: Human Monocyte Enrichment and Macrophage Differentiation: Mononucleus cells were isolated from peripheral blood of healthy adults (age = 18–40y) with human peripheral blood monocyte isolation Solution kit (Solarbio, P8680).

Techniques: Derivative Assay, Clinical Proteomics, Concentration Assay, Enzyme-linked Immunosorbent Assay, Standard Deviation, Imaging, Computed Tomography, Labeling, Immunostaining, Expressing, Cytometry

( A ) UMAP plot of monocyte clusters in WT lungs ( n = 3) and CLEC5A −/− lungs ( n = 3) and percentage in immune cells. ( B ) Dot plot showing the expression of adhesion molecules in each subtype of endothelial cells. ( C ) GSEA of the DEGs in the endothelial cells between CLEC5A −/− and WT lungs showing significant enrichment of cell-adhesion related-GO pathways, in which the up-regulated DEGs were mainly enriched. The significance was determined by P value with false discovery rate (FDR) < 0.25. ( D ) Mouse PMVECs were isolated and challenged by LPS (10 μg/ml) for 24 hours. ( E ) Levels of MCP-1 and VCAM-1 in the supernatant by ELISA. ( F ) Relative mRNA expression of MCP-1 and VCAM-1 in PMVECs. ( G ) Overview of in vitro adhesion and trans-endothelial migration assays in mouse PMVECs and HUVECs. ( H ) Mouse PBMCs were labeled by BCECF-AM, and the adherent monocytes to PMVECs were observed under a fluorescence microscopy. Scale bar, 50 μm. Monocyte-endothelial adhesion was quantified as the percentage of fluorescence intensity relative to the WT group. ( I ) Transmigration of mouse PBMCs across the PMVEC monolayer was shown as the percentage of transmigrated cells relative to the WT group by MTT assay. Lentivirus-mediated gene delivery for CLEC5A (CLEC5A oe ) or shRNA targeting CLEC5A (CLEC5A sh ) were carried out in HUVECs. ( J ) Adhesion of THP-1 to HUVECs was shown as fluorescence and quantified as the percentage of fluorescence intensity relative to the control group. Scale bar, 50 μm. ( K ) Migration of THP-1 through the HUVEC monolayer shown as the percentage of transmigrated cells relative to the control group. The statistical significance was determined by an unpaired two-tailed t test (or with Welch’s correction) for comparison between two groups and by one-way ANOVA test for comparisons among four groups (biological replicates, n = 4 per group for each experiment).

Journal: Science Advances

Article Title: Endothelial CLEC5A drives barrier dysfunction and vascular leakage responsible for lung injury in bacterial pneumonia and sepsis

doi: 10.1126/sciadv.adt7589

Figure Lengend Snippet: ( A ) UMAP plot of monocyte clusters in WT lungs ( n = 3) and CLEC5A −/− lungs ( n = 3) and percentage in immune cells. ( B ) Dot plot showing the expression of adhesion molecules in each subtype of endothelial cells. ( C ) GSEA of the DEGs in the endothelial cells between CLEC5A −/− and WT lungs showing significant enrichment of cell-adhesion related-GO pathways, in which the up-regulated DEGs were mainly enriched. The significance was determined by P value with false discovery rate (FDR) < 0.25. ( D ) Mouse PMVECs were isolated and challenged by LPS (10 μg/ml) for 24 hours. ( E ) Levels of MCP-1 and VCAM-1 in the supernatant by ELISA. ( F ) Relative mRNA expression of MCP-1 and VCAM-1 in PMVECs. ( G ) Overview of in vitro adhesion and trans-endothelial migration assays in mouse PMVECs and HUVECs. ( H ) Mouse PBMCs were labeled by BCECF-AM, and the adherent monocytes to PMVECs were observed under a fluorescence microscopy. Scale bar, 50 μm. Monocyte-endothelial adhesion was quantified as the percentage of fluorescence intensity relative to the WT group. ( I ) Transmigration of mouse PBMCs across the PMVEC monolayer was shown as the percentage of transmigrated cells relative to the WT group by MTT assay. Lentivirus-mediated gene delivery for CLEC5A (CLEC5A oe ) or shRNA targeting CLEC5A (CLEC5A sh ) were carried out in HUVECs. ( J ) Adhesion of THP-1 to HUVECs was shown as fluorescence and quantified as the percentage of fluorescence intensity relative to the control group. Scale bar, 50 μm. ( K ) Migration of THP-1 through the HUVEC monolayer shown as the percentage of transmigrated cells relative to the control group. The statistical significance was determined by an unpaired two-tailed t test (or with Welch’s correction) for comparison between two groups and by one-way ANOVA test for comparisons among four groups (biological replicates, n = 4 per group for each experiment).

Article Snippet: Mouse peripheral blood monocytes (PBMCs) were isolated and purified using the commercial kit (P5230#, Solarbio, Beijing, China).

Techniques: Expressing, Isolation, Enzyme-linked Immunosorbent Assay, In Vitro, Migration, Labeling, Fluorescence, Microscopy, Transmigration Assay, MTT Assay, shRNA, Control, Two Tailed Test, Comparison

CD3 + T cells acquire PEDV from DCs and enter the peripheral blood. a After PEDV intranasal inoculation, blood samples were collected at the indicated time during which PBMCs were isolated and analyzed by FACS. The cells were gated based on CD3 + T cells (Q1), and gated cells were further selected based on the uptake of PEDV. b Quantification of the FACS results is shown in panel ( a ), n = 6 from 3 piglets per group. c , d PEDV-pulsed DCs were cocultured with CD3 + T cells to detect the transmission of DC-associated PEDV to CD3 + T cells. Then, PEDV transmission was quantified by analyzing the CD3 + PEDV + population, n = 3 wells per group. e Furthermore, after coculturing with CD3 + T cells for 2 h, DCs were separated by magnetic beads separation (MACS). The remaining CD3 + T cells remained in culture, and PEDV RNA expression levels were determined at the indicated time; the results of a representative experiment are shown, n = 3 per group. f FACS profiles of conjugate formation between CFSE-labeled T cells ( x -axis) and PKH26-labeled PEDV-pulsed DCs ( y -axis). Conjugates are apparent in the upper right quadrant, and the percentages of cells are shown. g Conjugation between CFSE-labeled T cells (green) and PKH26-labeled DCs (red) is indicated by CLSM, and the typical conjugation structure between the two cells is enlarged in panel ( a ). PEDV (blue) was detected in the conjugate structure using an antibody against N protein. Bars, 20 μm. h Electron micrographs of DC-T cell conjugates. PEDV-carrying DCs were cocultured with CD3 + T cells for 1 h and collected for TEM analysis. The sites of interaction between DCs and T cells showed firm interactions (black frame). DCs and T cell membranes were closely apposed at the tips of the protrusions (black asterisk). The fine ultrastructure of the virus particles (black arrowheads) was observed in the T cells adjacent to the conjugate structure. Bars, 2 μm. All data are the mean ± SD, comparisons performed with one-way ANOVA. * P < 0.05. The results are representative of three independent experiments

Journal: Nature Communications

Article Title: An alternative pathway of enteric PEDV dissemination from nasal cavity to intestinal mucosa in swine

doi: 10.1038/s41467-018-06056-w

Figure Lengend Snippet: CD3 + T cells acquire PEDV from DCs and enter the peripheral blood. a After PEDV intranasal inoculation, blood samples were collected at the indicated time during which PBMCs were isolated and analyzed by FACS. The cells were gated based on CD3 + T cells (Q1), and gated cells were further selected based on the uptake of PEDV. b Quantification of the FACS results is shown in panel ( a ), n = 6 from 3 piglets per group. c , d PEDV-pulsed DCs were cocultured with CD3 + T cells to detect the transmission of DC-associated PEDV to CD3 + T cells. Then, PEDV transmission was quantified by analyzing the CD3 + PEDV + population, n = 3 wells per group. e Furthermore, after coculturing with CD3 + T cells for 2 h, DCs were separated by magnetic beads separation (MACS). The remaining CD3 + T cells remained in culture, and PEDV RNA expression levels were determined at the indicated time; the results of a representative experiment are shown, n = 3 per group. f FACS profiles of conjugate formation between CFSE-labeled T cells ( x -axis) and PKH26-labeled PEDV-pulsed DCs ( y -axis). Conjugates are apparent in the upper right quadrant, and the percentages of cells are shown. g Conjugation between CFSE-labeled T cells (green) and PKH26-labeled DCs (red) is indicated by CLSM, and the typical conjugation structure between the two cells is enlarged in panel ( a ). PEDV (blue) was detected in the conjugate structure using an antibody against N protein. Bars, 20 μm. h Electron micrographs of DC-T cell conjugates. PEDV-carrying DCs were cocultured with CD3 + T cells for 1 h and collected for TEM analysis. The sites of interaction between DCs and T cells showed firm interactions (black frame). DCs and T cell membranes were closely apposed at the tips of the protrusions (black asterisk). The fine ultrastructure of the virus particles (black arrowheads) was observed in the T cells adjacent to the conjugate structure. Bars, 2 μm. All data are the mean ± SD, comparisons performed with one-way ANOVA. * P < 0.05. The results are representative of three independent experiments

Article Snippet: Porcine PBMCs were isolated from the blood of piglets by density centrifugation using a porcine peripheral blood lymphocyte separation kit (Solarbio).

Techniques: Isolation, Transmission Assay, Magnetic Beads, RNA Expression, Labeling, Conjugation Assay, Virus